To observe the histological effects on the adrenal glands by the venoms of Agkistrodon snakes in Korea, freeze dried venom was administered to the rats with weight ranged 200-250gm. Each venom of A. b. brevicaudus (44mg), A. caliginosus (40mg) and A. saxatilis (42mg) was soluted in 12 ml of normal saline respectively and 0.4ml of which were administered through the tail vein of the each raft. I- Iistopathological observations on the adrenal glands were done sequentially with time interval
s4 after venom administration at 1 hr., 3 hrs., 6 hrs., 24 hrs., 4 days and 7 days, respectively.
ryF The results were as follows;
x 1. In A. b. brevicaudus intoxication, 15 out of 52 rats (28.85) were dead and revealed diffuse congestion in 11, focal hemorrhage in 5 and focal necrosis in 2 among them. In I to 6 hours were noted
r 10 diffuse congestion among 19 rats and focal hemorrhage in 2, diffuse hemorrhage in 2, and
} focal or diffuse necrosis in 3. In 24 hours were noted 3 focal necrosis in 7 rats. Only mild `congestion was noted in 4 to 7 days.
2. In A. caliginosus intoxication, 8 out of 57 rats (14.7%) were dead and revealed diffuse congestion 1 in 6, focal hemorrhage in 4 and focal necrosis in 2 among therm. In 1 to 6 hours were noted
6 diffuse congestion among 21 rats, and focal hemorrhage in 2. In 24 hours, were noted diffuse
congestion in 4 and focal necrosis in 1 among 10 rats. Diffuse congestion in 1 was noted among ~. 18 rats in 4 to 7 days.
3. In A. saxatilis intoxication, 8 out 50 rats (16%) were dead and revealed diffuse congestion in 7, focal hemorrhage in 1 and focal necrosis in 1 among them. In 1 to 6 hours were noted diffuse congestion in 8, focal hemorrhage in 3 and diffuse necrrosis in 1 among 25 rats. In 24, hours diffuse congestion in 1 and focal necrosis in 3 among 7 rats. Diffuse congestion in 2 among 10 rats in 4 to 7 days.
Therefore, it was suggested that all three kinds of Agkistrodon snake venom in Korea could induce diffuse congestion, hemorrhage and/or necrosis in adrenal glands by its hematoxic character of the venom.
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